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CAS RN: 1239-45-8 | 产品编码: E1363
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
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Product Information
Volume : 10 mLDescription
This product contain 0.5mg/mL Ethidium Bromide and can use as nucleic acid stain solution or electrophoresis buffer solution. Each drop contains 20 µg of Ethidium Bromide, so you can easily adjust the solution as final concentration. Convenient and safe to use because of dropper bottle.
Storage
The product should be stored at 4 °C, protect from light.
Description for Use
After electrophoresis, dilute an Ethidium Bromide solution to 0.5 μg/mL with water or running buffer and incubate the gel for 15-30 min. Destaining is usually not needed. If you have to decrease background fluorescence, wash the gel in water for 15 minutes. In use of electrophoresis buffer solution, Ethidium Bromide incorporated into nucleic acid and can visualize band immediately by using UV transilluminator.
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技术规格
| Appearance | Light yellow to Yellow to Orange clear liquid |
| DNA staining test | to pass test |
物性（参考值）
GHS
相关法规
| RTECS# | SF7950000 |
运输信息
| HS编码* | 2933.99-000 |
应用
DNA Staining Reagents for Agarose Gel Electrohporesis
Figure 1. Agarose gel after staining with ethidium bromide (0.5 mg/mL solution) (taken in-house) 
Figure 2. Agarose gel after staining with 10X Nucleic Acid Stain Blue (taken in-house)
References
- Agarose Gel Electrophoresis for the Separation of DNA Fragments
- Detection of DNA in Agarose Gels
- J. F. Sambrook, D. W. Russell, in Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, New York, 2001, Vol. 1, Chap. 5, 14.
应用
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
References
-
- Cold Spring Harbor Laboratory Press, New York
- Joseph Sambrook and David W. Russell., Molecular Cloning third edition, A Laboratory Manual, 2001, 5. 14.
- Importance of Binding Affinity for the Activity of a Metallodendritic Chemical Nuclease.
- Efficient removal of ethidium bromide from aqueous solution by using DNA-loaded Fe3O4 nanoparticles
- Cold Spring Harbor Laboratory Press, New York
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