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CAS RN: 28718-90-3 | 产品编码: D5888
DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
![DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining] No-Image](/medias/D5888.jpg?context=bWFzdGVyfHJvb3R8MjkxNzJ8aW1hZ2UvanBlZ3xhREpoTDJneE15ODVNVE13TVRJMU5qZzRPRFl5TDBRMU9EZzRMbXB3Wnd8NTViZjJjMzY3ZmEyMzI5NTkzNTQ3OGMxMjE0YjJiYmYwNTkyMjFmMTAzMDVkMTdkYzllMzVlMWZlMjBkMWUzYg)
![DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining] No-Image](/medias/Tci-440-D5888-Nuclei-of-HeLa-cells-stained-by-DAPI-2HCl.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8Nzc4MDN8aW1hZ2UvanBlZ3xhRGc1TDJnM09TODVOVEUwTVRNek1ESTRPRGswTDFSamFTMDBOREJmUkRVNE9EZ3RUblZqYkdWcExXOW1MVWhsVEdFdFkyVnNiSE10YzNSaGFXNWxaQzFpZVMxRVFWQkpMVEpJUTJ3dWFuQm58YzVjYWRjZjU2YjJlNjliM2U4NDAxZmU4NDJiN2ViZDhiYWY2N2EwZjMwYmU1ZjJlOTIzYmFmODkzZDM1NWJjYQ)
纯度/分析方法:
别名:
- DAPI 2HCl (0.2mL×5) (1mg/mL于水中) [用于细胞染色]
- 4',6-二脒基-2-苯基吲哚二盐酸盐 (0.2mL×5) (1mg/mL于水中)
- 2-(4-氨基甲酰基苯基)-1H-吲哚-6-羧酰胺二盐酸盐 (0.2mL×5) (1mg/mL于水中)
- 6-脒基-2-(4-脒基苯基)吲哚二盐酸盐 (0.2mL×5) (1mg/mL于水中)
- 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
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Product Information
- Volume : 0.2 mL x 5 vial
- Concentration : 1.0 mg/mL
Description
- The DAPI is a fluorescent stain for labeling DNA.
DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).
Description for Use
- Fluorescence Staining of Cell Nucleus
- Fix and permeabilize cells as desired.
- Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
*Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL. - Incubate with cells at room temperature for 5-20 minutes (**).
**Extend Incubate time as desired. - Mount sample with an appropriate medium and proceed to imaging.
Storage
- Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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技术规格
Appearance | Light yellow to Yellow clear liquid |
DNA staining test | pass test |
物性(参考值)
最大吸收波长 | 342(H2O) nm |
GHS
相关法规
RTECS# | NL5995054 |
运输信息
HS编码* | 2933.99-000 |
应用
Fluorescent staining of DNA in cells
References
- Binding of 4',6-diamidino-2-phenylindole (DAPI) to AT Regions of DNA: Evidence for an Allosteric Conformational Change
- Photoconversion of DAPI and Hoechst dyes to green and red emitting forms after exposure to UV excitation
- Protein kinase C involvement in aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell
- Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions
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