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CAS RN: 28718-90-3 | 产品编码: D5888

DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]


纯度/分析方法:
别名:
  • DAPI 2HCl (0.2mL×5) (1mg/mL于水中) [用于细胞染色]
  • 4',6-二脒基-2-苯基吲哚二盐酸盐 (0.2mL×5) (1mg/mL于水中)
  • 2-(4-氨基甲酰基苯基)-1H-吲哚-6-羧酰胺二盐酸盐 (0.2mL×5) (1mg/mL于水中)
  • 6-脒基-2-(4-脒基苯基)吲哚二盐酸盐 (0.2mL×5) (1mg/mL于水中)
  • 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
  • 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
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Product Information

  • Volume : 0.2 mL x 5 vial
  • Concentration : 1.0 mg/mL

Description

  • The DAPI is a fluorescent stain for labeling DNA.
    DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).

Description for Use

  • Fluorescence Staining of Cell Nucleus
    1. Fix and permeabilize cells as desired.
    2. Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
      *Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL.
    3. Incubate with cells at room temperature for 5-20 minutes (**).
      **Extend Incubate time as desired.
    4. Mount sample with an appropriate medium and proceed to imaging.

Storage

  • Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Related Products

  • A2412 DAPI·2HCl [for Biochemical Research]
  • B6236 Bisbenzimide H 33258 (1mg/mL in Water) (0.2mL*5) [for Cell Staining]
  • E1363 Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
  • G0406 Goat Anti-Mouse IgG FITC Conjugate
  • G0452 Goat Anti-Rabbit IgG FITC Conjugate
  • G0453 Goat Anti-Mouse IgM FITC Conjugate
  • G0569 Goat Anti-Mouse IgG R-PE Conjugate
  • G0577 Goat Anti-Rabbit IgG R-PE Conjugate
  • H1343 Bisbenzimide H 33258 Hydrate [for Biochemical Research]
  • S0966 Streptavidin FITC Conjugate
  • T3885 Streptavidin R-PE Conjugate

产品编码 D5888
分子式/分子量 C__1__6H__1__5N__5·2HCl = 350.25 
外观与形状(20°C) 液体
储存温度 冷冻 (<0°C)
应避免的情况 光,加热
CAS RN 28718-90-3
Reaxys-RN 4894417
PubChem物质ID 468591628
MDL编号

MFCD00012681

技术规格
Appearance Light yellow to Yellow clear liquid
DNA staining test pass test
物性(参考值)
最大吸收波长 342(H2O) nm
GHS
相关法规
RTECS# NL5995054
运输信息
HS编码* 2933.99-000
*此H.S.编码用于日本出口报关,不适用于您所在国家或地区的进口申报
应用
Fluorescent staining of DNA in cells

References


TCIMail
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技术规格
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