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CAS RN: | 产品编码: M3353

MTT Solution [for Cell proliferation assay] (1mL×5)


纯度/分析方法:
别名:
  • MTT溶液 [用于细胞增殖检测] (1mL×5)
  • 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑-3-鎓溴化物溶液 (1mL×5)
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide Solution (1mL×5)
产品文档:
1SET
S$120.00
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补充产品信息:

Product Information

  • Volume/Quantity : 1mL x 5
  • Product Form : 5.0 mg/mL in PBS

Description

  • 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a tetrazolium salt that is converted to formazan when taken up by living cells. Although formazan forms crystals in cells due to its insolubility in water, it is soluble in DMSO. By dissolving these formazan crystals in DMSO and reading the resultant absorbance at 540 nm, an estimate of cell number can be obtained. This product comprises a solution of 5.0 mg/mL MTT dissolved in PBS. To use, simply apply 1/10 of the culture medium volume of MTT Solution to live cells.

Storage

  • Stored at -20 °C and protected from light. Avoid repeated freezing and thawing.

Direction for Use

  • MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 5,000 to 50,000 cells per well in a 96-well plate.
    1. Seed 100 µL of cells in a 96-well plate.
    2. Incubate overnight in an incubator.
    3. Add 10 µL of MTT solution to each well.
    4. Incubate for 2-4 hours for color reaction.
    5. Remove the medium, taking care not to suck up the formazan.
    6. Add 100 µL of DMSO (Product No. D0798) and dissolve the formazan.
    7. Measure the absorbance at 540 nm.

Related Products

  • R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
  • D0798 Dimethyl Sulfoxide
  • M3297 MTT [for Biochemical Research]

产品编码 M3353
外观与形状(20°C) 液体
储存温度 冷冻 (-20°C)
应避免的情况 加热
技术规格
Appearance Light yellow to Yellow to Orange clear liquid
Cell staining test to pass test (min. 0.5, Abs at 540nm)
物性(参考值)
GHS
相关法规
运输信息
HS编码* 3822.19-000
*此H.S.编码用于日本出口报关,不适用于您所在国家或地区的进口申报
产品文档 (部分产品的分析图谱无法提供,敬请谅解。)
化学品安全说明书(SDS)
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技术规格
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