MTT Solution [for Cell proliferation assay] (1mL×5)

×

纯度/分析方法:

别名：

• MTT溶液 [用于细胞增殖检测] (1mL×5)
• 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑-3-鎓溴化物溶液 (1mL×5)
• 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide Solution (1mL×5)

产品文档：

SDS | 技术规格 | CoA及其他文档 | 分析图谱

基本信息 | 技术规格和文件 | 安全和法规 | 应用实例・文献

补充产品信息

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1SET	S$120.00	≥60	0	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

添加到您的购物车

添加到购物车

大包装询价

Product Information

• Volume/Quantity : 1mL x 5
• Product Form : 5.0 mg/mL in PBS

Description

• 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a tetrazolium salt that is converted to formazan when taken up by living cells. Although formazan forms crystals in cells due to its insolubility in water, it is soluble in DMSO. By dissolving these formazan crystals in DMSO and reading the resultant absorbance at 540 nm, an estimate of cell number can be obtained. This product comprises a solution of 5.0 mg/mL MTT dissolved in PBS. To use, simply apply 1/10 of the culture medium volume of MTT Solution to live cells.

Storage

• Stored at -20 °C and protected from light. Avoid repeated freezing and thawing.

Direction for Use

• MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 5,000 to 50,000 cells per well in a 96-well plate.

• 1. Seed 100 µL of cells in a 96-well plate.
  2. Incubate overnight in an incubator.
  3. Add 10 µL of MTT solution to each well.
  4. Incubate for 2-4 hours for color reaction.
  5. Remove the medium, taking care not to suck up the formazan.
  6. Add 100 µL of DMSO (Product No. D0798) and dissolve the formazan.
  7. Measure the absorbance at 540 nm.

Related Products

• R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
• D0798 Dimethyl Sulfoxide
• M3297 MTT [for Biochemical Research]