Maximum quantity allowed is 999
CAS RN: 65-61-2 | 产品编码: A3396
Acridine Orange Solution [for Cell Staining]
补充产品信息:
Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution
Description
Acridine orange is a cell membrane-permeant nucleic acid cell stain, which is incorporated into double-stranded DNA at a ratio of one to three base pairs. It fluoresces at 520nm (Ex. 500nm) when bound to double stranded DNA, and 650nm (Ex. 460nm) when bound to RNA or single-stranded DNA. As acridine orange is mutagenic, this product is supplied as a pre-made solution to prevent exposure during weighing.
Direction for Use
Adjust volume and staining time as necessary. Because some cells need to be fixed prior to staining, preliminary testing should be performed.
1. Remove medium from cells and wash twice with cold PBS(-).
2. Remove PBS(-) and add an appropriate volume of 1:50 Acridine Orange (Product No. A3396) in PBS(-). Allow to stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Observe cells with a fluorescence microscope while submerged in PBS(-).
Storage
Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months.
Related Products
R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
R0246 RIPA Buffer (Ready-to-use) [for Protein extraction]
O0483 Oil Red O [for Biochemical Research]
产品编码 | A3396 |
分子式/分子量 | C__1__7H__1__9N__3·HCl = 301.82 |
外观与形状(20°C) | 液体 |
储存温度 | 冷藏 (0-10°C) |
应避免的情况 | 光,加热 |
CAS RN | 65-61-2 |
PubChem物质ID | 468590276 |
MDL编号 | MFCD00012660 |
Appearance | Light yellow to Brown clear liquid |
DNA staining test | to pass test |
RTECS# | AR7601000 |
HS编码* | 3204.19-900 |
References
- The frequencies of micronuclei induced by cisplatin in newborn rat astrocytes are increased by 50-Hz, 7.5- and 10-mT electromagnetic fields
- Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA
化学品安全说明书(SDS)
如需更多帮助,请联系我 们。
技术规格
CoA及其他文档
示例 CoA
目前没有该产品的 CoA 示例。
分析图谱
很抱歉,您搜索的分析图谱无法提供。