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CAS RN: | 产品编码: T3854

TMB [for ELISA] (Ready-to-use solution)

× TMB [for ELISA] (Ready-to-use solution)

纯度/分析方法:
别名：
  • TMB [用于ELISA] (即用型溶液)
  • 3,3',5,5'-四甲基联苯胺 [用于ELISA可溶性显色底物] (即用型溶液)
  • 3,3',5,5'-Tetramethylbenzidine [Soluble chromogenic substrate for ELISA] (Ready-to-use solution)
产品文档：
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100ML
S$130.50
 ≥40  16   请联系我们
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补充产品信息:

Product Information
Volume : 100 mL

Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.

Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.

Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.

Related Products
T3855 TMB (Ready-to-use solution) [for Western blotting]
T2573 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]
H1202 Hydrochloric Acid (1mol/L)
H1621 Horseradish Peroxidase Maleimide Conjugate
A3075 Anti-6xHis Monoclonal Antibody (6A12) HRP Conjugate
A3187 Anti-Protein A Chicken Polyclonal Antibody HRP Conjugate
A3195 Anti-α Gal Chicken Polyclonal Antibody HRP Conjugate
G0407 Goat Anti-Mouse IgG HRP Conjugate
G0417 Goat Anti-Mouse IgM HRP Conjugate
G0418 Goat Anti-Rabbit IgG HRP Conjugate
P2466 Protein A HRP Conjugate
S0972 Streptavidin HRP Conjugate
S0999 Sheep Anti-Chicken IgY HRP Conjugate

产品编码 T3854
外观与形状（20°C） 液体
储存温度 冷藏 (0-10°C)
应避免的情况 加热
技术规格
Appearance Colorless to Almost colorlessclear liquid
Peroxidase detection test to pass test
物性（参考值）
GHS
象形图 Pictogram
信号词 Danger
危险性说明 H370 : Causes damage to organs.
H372 : Causes damage to organs through prolonged or repeated exposure.
H227 : Combustible liquid.
防范说明 P501 : Dispose of contents/ container to an approved waste disposal plant.
P270 : Do not eat, drink or smoke when using this product.
P260 : Do not breathe mist or vapours.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.
P403 : Store in a well-ventilated place.
P405 : Store locked up.
相关法规
运输信息
HS编码* 3822.19-000
*此H.S.编码用于日本出口报关，不适用于您所在国家或地区的进口申报
应用
TMB [for ELISA] (Ready-to-use solution)

References

  • Cold Spring Harbor Laboratory Press, New York
    • E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594


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