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CAS RN: | 产品编码: N1109
4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
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补充产品信息:
Product Information
Volume : 100 mL
Preservative : 0.02% Sodium azide
Description
This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears. After terminating the reaction by adding NaOH solution, the absorbance of reaction product is measured at 405 nm.
Application
The following steps are for a 96-well plate format.
1. Bring pNPP solution (Product No. N1109) to room temperature.
2. Gently mix the pNPP solution (Product No. N1109).
3. Add 100uL of pNPP solution (Product No. N1109) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100 uL of 1 N NaOH (Product No. S0542) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 405 nm.
Storage
The product should be stored at 4 ºC and used within 6 months from purchase.The product may be frozen but this does not affect its quality; thaw it at room temperature before use.
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S0542 Sodium Hydroxide (1mol / L in Water)
| 产品编码 | N1109 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 冷冻 (-20°C) |
| 应避免的情况 | 加热 |
技术规格
| Appearance | Colorless to Light orange to Yellow clear liquid |
| Alkaline Phosphatase detection test | to pass test |
物性（参考值）
GHS
| 象形图 |
|
| 信号词 | Danger |
| 危险性说明 | H302 : Harmful if swallowed. H315 : Causes skin irritation. H318 : Causes serious eye damage. H361 : Suspected of damaging fertility or the unborn child. H371 : May cause damage to organs. H373 : May cause damage to organs through prolonged or repeated exposure. H334 : May cause allergy or asthma symptoms or breathing difficulties if inhaled. H351 : Suspected of causing cancer. H402 : Harmful to aquatic life. |
| 防范说明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P273 : Avoid release to the environment. P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray. P270 : Do not eat, drink or smoke when using this product. P202 : Do not handle until all safety precautions have been read and understood. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P284 : Wear respiratory protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P362 + P364 : Take off contaminated clothing and wash it before reuse. P304 + P340 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P301 + P312 + P330 : IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth. P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. P405 : Store locked up. |
相关法规
运输信息
| HS编码* | 3822.19-000 |
应用
4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
References
- Antibodies, A Laboratory Manual, ed. by E. Harlow, D. Lane, Cold Spring Harbor Laboratory, New York, 1988, p. 597.
- Enzyme immunoassays in diagnostic medicine
- A. Voller, D. E. Bidwell, A. Bartlett, Bull. World Health Organ. 1976, 53, 55-65.
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