RIPA Buffer (Ready-to-use) [for Protein extraction]

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補充產品訊息

包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
100ML	NT$3,160	34	0	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information

• Volume : 100 mL
• Additives : WITHOUT protease inhibitors

Description

• This product is supplied as a ready-to-use solution to lyse cultured mammalian cells. The extraction from cultured cells by RIPA buffer (Product No. R0246) can be transferred directly to further analysis. This product does not include protease inhibitors, thus please add protease inhibitor cocktail to the product as necessary.

Direction for Use: Lysis of adherent cells

• 1. Remove medium from cultured cells and wash cells twice with cold PBS.
  2. Remove PBS and add cold RIPA buffer (Product No. R0246) to the cells. 1 mL of RIPA buffer is added to 0.5 - 5.0 x 10⁷ cells.
  3. Scrape the adherent cells with a cell scraper and transfer the lysate and pellet to a tube.
  4. Incubate the cells for 15 minutes on ice. (To increase the yields, prolong the incubation.)
  5. Centrifuge the tube at 10,000 x g for 10 minutes at 4 °C.
  6. Transfer the supernatant to a new tube for further analysis.

Direction for Use: Lysis of suspension cells

• 1. Transfer the cultured cells to a tube, remove medium from cultured cells and wash cells twice with cold PBS.
  2. Remove PBS and add cold RIPA buffer (Product No. R0246) to the cells. 1 mL of RIPA buffer is added to 0.5 - 5.0 x 10⁷ cells.
  3. Incubate the cells for 15 minutes on ice. (To increase the yields, prolong the incubation.)
  4. Centrifuge the tube at 10,000 x g for 10 minutes at 4 °C.
  5. Transfer the supernatant to a new tube for further analysis.

Storage

• The product should be stored at 4 °C and used within 6 months.

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