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CAS RN: | Product Number: N1113
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
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Purity:
Synonyms:
- NBT / X-Phosphate p-Toluidine Salt Solution (50X)
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 5ML |
NT$2,760
|
10 | 0 | Contact Us |
* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact
our distributor
in Taiwan to order our product.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
Volume : 5 mL
Description
This product is supplied as a concentrated solution containing Nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.
Direction for Use
1: Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
2: Dilute NBT/ X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl)
3: Incubate the washed membrane with 1X NBT/ X-Phosphate p-Toluidine Salt solution until color development.
4: Add deionized water to stop the color development.
Storage
The product should be stored at 4 ºC. Don’t freeze because of arising precipitate.
Related Products
B1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro blue tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
| Product Number | N1113 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
Specifications
| Appearance | Yellow to Amber to Dark purple clear liquid |
| Alkaline Phosphatase detection test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H373 : May cause damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P314 : Get medical advice/ attention if you feel unwell. P403 : Store in a well-ventilated place. |
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p407, p505, p598
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