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CAS RN: | 产品编码: W0024

Western Blot Stripping Buffer [for Biochemical Research]


纯度/分析方法:
别名:
  • 蛋白质印迹剥离缓冲液 [用于生化研究]
产品文档:
250ML
S$88.50
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Product Information

  • Volume : 250 mL

Description

  • Western Blot Stripping Buffer is a reagent used for removing antibodies from membranes that detects chemiluminescence in Western blotting. After chemiluminescent detection, membranes treated with Stripping Buffer can be reused for antibody concentration studies or for re-detection with a different primary antibody. The Stripping Buffer is useful for saving samples and shortening the experiment time.
    Note: Dissolve the precipitation at 25-30 °C, if precipitation occurs during storage. The Stripping Buffer is acidic and corrosive. Please wear protective equipment when using it. Metal tweezers and trays should not be used with the Stripping Buffer.

Storage

  • Store at room temperature.

Direction for Use

    1. After detecting chemiluminescence, wash the membrane twice for 10 minutes with TBS-T or PBS-T buffer.
    2. Incubate the membrane with the Stripping Buffer for 30 minutes at room temperature. Use a sufficient volume of the Stripping Buffer to ensure that the membrane is completely submerged.
      Note: Depending on the affinity between the antigen and the antibody, the antibody may not be completely removed from the membrane.
    3. Wash the membrane three times for 10 minutes each with TBS-T or PBS-T buffer.
    4. Perform blocking and antigen-antibody reaction again.

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产品编码 W0024
外观与形状(20°C) 液体
储存温度 室温 (15°C以下阴凉干燥处)
技术规格
Appearance Colorless to Almost colorlessclear liquid
Functionality test to pass test
物性(参考值)
比重 1.00
GHS
象形图 Pictogram
信号词 Warning
危险性说明 H373 : May cause damage to organs through prolonged or repeated exposure.
防范说明 P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P314 : Get medical advice/ attention if you feel unwell.
相关法规
运输信息
HS编码* 3822.19-000
*此H.S.编码用于日本出口报关,不适用于您所在国家或地区的进口申报
产品文档 (部分产品的分析图谱无法提供,敬请谅解。)
化学品安全说明书(SDS)
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技术规格
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