CAS RN: | Product Number: B6104

4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

Purity:

Synonyms:

Product Documents:

Size	Unit Price	Belgium	Japan*	Quantity	Shipping Information
20ML	€ 36,00	Contact Us	≥40		Shipping Simulation

* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)

Added to Your Shopping Cart

Supplemental Product Information:

Product Information

Volume : 20 mL

Description

This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

Preparation of non-reducing sample
A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 20 μL of 2-Mercaptoethanol per 80 μL of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95–100°C for 5 minutes. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

Store at room temperature.

Related Products

A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6105 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0615 Gel Negative Stain kit [for Electrophoresis]
I1309 Silver Stain Kit [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]

General Information plus

Specifications & Properties plus

Specifications

Appearance	Purple to Dark purple clear liquid to cloudy liquid
Functionality test	Electrophoresis to pass test

Properties (reference)

Specific Gravity (20/20)

1.14

Safety & Regulations plus

GHS

Pictogram
Signal Word	Danger
Hazard Statements	H318 : Causes serious eye damage. H371 : May cause damage to organs.
Precautionary Statements	P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P264 : Wash skin thoroughly after handling. P280 : Wear eye protection/ face protection. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor.