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CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
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Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days |
|---|---|---|---|
| 100ML |
A$215.00
|
4 | 2 |
* Please contact our distributor of Chem-Supply
if you would like to purchase TCI products. The above prices do not include freight cost, customs charge and other charges to the destination.
* ChemSupply Australia Pty Ltd (Phone: 08-8440-2000 / email: sales@chemsupply.com.au)
* ChemSupply Australia Pty Ltd (Phone: 08-8440-2000 / email: sales@chemsupply.com.au)
Supplemental Product Information:
Product Information
Volume : 100 mLAdditives : WITHOUT protease inhibitors
Description
This product is supplied as a ready-to-use solution to lyse cultured E.coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Directions for Use: Lysis of E.coli cells
1. Centrifuge E.coli cells at 5000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Incubate for 10 minutes at room temperature.
5. Centrifuge lysate at 15,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
1. Centrifuge yeast cells at 3000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Agitate at room temperature for 10 minutes.
5. Centrifuge lysate at 14,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Storage
Store at 4 °C and use within 6 months.
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| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Articles/Brochures
TCIMAIL
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
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