Nile Red Excellent at Detecting Lipid Droplets

Nile Red (1) is a lipophilic fluorescent dye with high photostability. It emits almost no fluorescence in water, but 1 emits strong fluorescence (Ex_max: 551 nm, Em_max: 631 nm) upon binding to hydrophobic regions of proteins or lipid droplets.¹⁾ 1 can distinctly stain lipid droplets in both live and fixed cells, making it widely used for detecting intracellular lipid droplets using fluorescence microscopy or flow cytometry.²⁾

Related Products

N1272

Nile Red [for Biochemical Research]

Related Product Category Pages

• Fluorescent Probes
• Fluorescent Lipid Droplet Stains

References

• 1) Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
  • D. L. Sackett, J. Wolff, Anal. Biochem. 1987, 167, 228.
• 2) Nile red: a selective fluorescent stain for intracellular lipid droplets
  • P. Greenspan, E. P. Mayer, S. D. Fowler, J. Cell Biol. 1985, 100, 965.

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