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Polyamines Suitable for Protein Stabilization
Polyamines are aliphatic compounds containing two or more amino groups per molecule, of which spermine and spermidine are typical examples. Polyamines are found ubiquitously throughout the domains of life, in cells ranging from microorganisms up to higher animals and plants. In recent years, the presence of high intracellular concentrations of polyamines has been linked to increased proliferative potential in cancer cells – opening the door to new therapies aimed at reducing intracellular polyamine concentrations.1) Polyamines are also useful as stabilizers of biomolecules; they have been reported to inhibit the heat-induced aggregation of lysozyme.2) Salts of spermine and spermidine, 1 and 2, have been confirmed to stabilize proteins (as shown in the below figure) and are effective at preventing aggregation of target proteins.
Figure. Residual activity of lysozyme after heating at 98 °C (in-house analysis).
Addition of 1 or 2 at a concentration of 100 mM allowed for over 80% of initial activity to be retained.
Related Products
- P2950
- Spermine Tetrahydrochloride [for Protein Research]
- P2957
- Spermidine Phosphate [for Protein Research]
Related Product Category Pages
References
- 1) Polyamine metabolism and cancer: treatments, challenges and opportunities
- 2) Prevention of thermal inactivation and aggregation of lysozyme by polyamines
