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CAS RN: | Product Number: R0241
rEGCase I assisted by Activator II
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Purity:
Synonyms:
- Recombinant Endoglycoceramidase I assisted by Activator II
- Recombinant - Rhodococcus equi EGCase I expressed in Rhodococcus erythropolis
- Recombinant Endoglycoceramidase I with Activator II
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1VIAL |
$473.00
|
Contact Us | 1 | 7 |
|
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Supplemental Product Information:
Product Information
- Quantity : 300 mU per vial (3 mU/μL) and 50 nmol Activator II per vial (0.5 nmol/μL)
- Product Form : Purified recombinant EGCase I and Activator II, Liquid
- Storage buffer : 20 mM Sodium phosphate, 50 mM NaCl buffer, pH 7.0
- Preservative : None
- Stabilizer : None
Description
- Endoglycoceramidase I (EGCase I) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under a detergent-containing condition. On the other hand, Activator II is capable of inducing activity of EGCase without any detergent reagents. This product is provided as a mixture-form of EGCase I and Activator II. By using this product, cell-surface glycosphingolipids of living cells are hydrolyzed without cell disruption caused by addition of detergent. The EGCase I gene is derived from Rhodococcus equi and its recombinant protein is expressed in Rhodococcus erythropolis.
Unit Definition
- One unit (U) of EGCase I is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.
Assay Conditions
- Reaction conditions: 50 mM sodium acetate, pH 5.5
The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL (without detergent). The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference). - [Reference]
Determination of Glucose by a Modification of Somogyi-Nelson Method
C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.
- Regarding hydrolysis of cellular GSLs, see the reference (Methods Mol. Biol.) below.
- [Reference]
Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi
M. Ito, Y. Ishibashi, T. Watanabe, J. Iwaki, T. Kurita, N. Okino, Methods Mol. Biol. 2023, 2613, 229.
Storage
- Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
Related Products
- G0483 Ganglioside GM1
- P1775 TRITON™ X-100
- Other oligosaccharide-releasing enzymes
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
| Product Number | R0241 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Titer | min. 300 mU/Vial |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3507.90.7000 |
Application
Endoglycoceramidase
References
- Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities
- Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system
Articles/Brochures
TCIMAIL
[TCIMAIL No.185] Endoglycoceramidase (EGCase): Glycosphingolipid-hydrolyzing Enzyme[Glycoscience Products] Endoglycoceramidase ― Hydrolytic Enzymes Specific to Glycosphingolipids
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