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CAS RN: | Product Number: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 10ML |
$62.00
|
1 | Contact Us | ≥60 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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Supplemental Product Information:
Product Information
Volume : 10 mLDescription
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.
Storage
Store at room temperature.
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B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
| Product Number | B6105 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Green to Dark green to Dark blue clear liquid to cloudy liquid |
| Functionality test | to pass test(Electrophoresis) |
Properties (reference)
| Specific Gravity (20/20) | 1.16 |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse. |
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
- Dodecyl sulfate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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