Ready-to-use 4-Nitrophenyl Phosphate Solution for ELISA

1 is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When 1 reacts with alkaline phosphatases, yellow colored soluble products appear. After terminating the reaction by adding NaOH aqueous solution, the absorbances of the reaction product are measured at 405 nm.

Direction for Use
The following steps are for a 96-well plate format.
1. Bring 1 to room temperature and mix it gently.
2. Add 100 μL of 1 to each well.
3. Incubate the plate at room temperature for 30 minutes.
4. To terminate the reaction, add 100 μL of 1 mol/L NaOH to each well.
5. Within 1 hour of terminating the reaction, measure the absorbance of each well at 405 nm.

Figure 1. Example of use with a 96-well plate