Make sure to sign up for an account today for exclusive coupons and free shipping on orders over $75!
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: N1113
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_pdp.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_mobile.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_thumbnail.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
×
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
Purity:
Synonyms:
- NBT / X-Phosphate p-Toluidine Salt Solution (50X)
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 5ML |
$117.00
|
Contact Us | Contact Us | 10 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
Volume : 5 mLDescription
This product is supplied as a concentrated solution containing nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.
Direction for Use
- Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
- Dilute NBT / X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl) - Incubate the washed membrane with 1X NBT / X-Phosphate p-Toluidine Salt solution until color development.
- Add deionized water to stop the color development.
Storage
Store at 4 °C. Do not freeze.
Related Products
D5909 DAB staining kitB1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro Blue Tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
| Product Number | N1113 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
Specifications
| Appearance | Yellow to Amber to Dark purple clear liquid |
| Alkaline Phosphatase detection test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H373 : May cause damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P210 : Keep away from heat/sparks/open flames/hot surfaces. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P314 : Get medical advice/ attention if you feel unwell. P403 + P235 : Store in a well-ventilated place. Keep cool. |
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p407, p505, p598
Articles/Brochures
TCIMAIL
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.