Sialidase (Neuraminidase) Agarose

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Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity
1VIAL	$450.00	Contact Us	Contact Us	5

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Product Information

• Preparation : Sialidase is covalently coupled with agarose gel
• Quantity : 0.1 mL gel/0.2 mL (1:1, v/v slurry) suspended in Storage buffer
• Activity : >10 U/mL gel
• Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
• Preservative : 0.02% [w/v] Sodium azide
• Stabilizer : None

Description

• The Sialidase Agarose is an enzyme-immobilized agarose.
  This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N- acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme-immobilized agarose and Neu5Ac-α-4MU in 200 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at 4°C. (Avoid freezing).

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