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CAS RN: 15687-27-1 | Product Number: I0415

Ibuprofen

Purity: >98.0%(GC)(T)

Synonyms:

2-(4-Isobutylphenyl)propionic Acid
4-Isobutyl-α-methylphenylacetic Acid

Product Documents:

Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*
25G	$69.00	13	3	≥100
100G	$166.00	5	1	9
500G	$498.00	Contact Us	6	15

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* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

General Information plus

Product Number	I0415
Purity / Analysis Method	>98.0%(GC)(T)
Molecular Formula / Molecular Weight	C₁₃H₁₈O₂ = 206.29
Physical State (20 deg.C)	Solid
Storage Temperature	Room Temperature (Recommended in a cool and dark place, <15°C)
CAS RN	15687-27-1
Reaxys Registry Number	2049713
PubChem Substance ID	87571768
SDBS (AIST Spectral DB)	10570
Merck Index (14)	4881
MDL Number	MFCD00010393

Specifications & Properties plus

Safety & Regulations plus

Applications & Literature plus

Application

Ibuprofen: A Non-Selective Non-Steroidal Anti-Inflammatory Drug (NSAID)

Ibuprofen is a non-selective non-steroidal anti-inflammatory drug (NSAID) in the propionic acid derivatives group, which possesses analgesic and antipyretic activities. Ibuprofen is a racemic compound and the active enantiomer is (S)-(+)-ibuprofen [I0549] which works by inhibiting both the COX-1 and COX-2 enzymes and prostaglandin synthesis. NSAIDs also include ketoprofen [K0038], loxoprofen [L0244] and naproxen [M1021] among others. Resently, some studies have suggested that NSAIDs may reduce the risk of certain types of cancer and Alzheimer's disease. (The product is for research purpose only.)

References

Ibuprofen: pharmacology, efficacy and safety (a review)
- K. D. Rainsford, Inflammopharmacology 2009, 17, 275.
Enantioselective pharmacokinetics of ibuprofen and involved mechanisms (a review)
- H. Hao, G. Wang, J. Sun, Drug Metab. Rev. 2005, 37, 215.
Cyclo-oxygenase-2: pharmacology, physiology, biochemistry and relevance to NSAID therapy (a review)
- J. A. Mitchell, T. D. Warner, Br. J. Pharmacol. 1999, 128, 1121.
Aspirin, ibuprofen, and other non-steroidal anti-inflammatory drugs in cancer prevention: A critical review of non-selective COX-2 blockade (a review)
- R. E. Harris, J. Beebe-Donk, H. Doss, D. B. Doss, Oncol. Rep. 2005, 13, 559.
NSAID and antioxidant prevention of Alzheimer's disease: Lessons from in vitro and animal models (a review)
- G. M. Cole, T. Morihara, G. P. Lim, F. Yang, A. Begum, S. A. Frautschy, Ann. N.Y. Acad. Sci. 2004, 1035, 68.
Resolution of enantiomers of ibuprofen by liquid chromatography: a review
- R. Bhushan, J. Martens, Biomed. Chromatogr. 1998, 12, 309.
Ibuprofen (a review on the methods of preparations, physical properties, stability, metabolism and pharmacokinetics and methods for the detection of ibuprofen)
- J. D. Higgins, T. P. Gilmor, S. A. Martellucci, R. D. Bruce, H. G. Brittain, Anal. Profiles Drug Subst. Excipients 2001, 27, 265.

Application

Binding of Ibuprofen to Human Serum Albumin

Ibuprofen is known to have affinity for Human Serum Albumin (HSA) and to bind (interact) to drug binding site II on HSA. Those were confirmed using our ibuprofen with Surface Plasmon Resonance (SPR) and a method using fluorescent probes. 【SPR】Dose responses of ibuprofen to HSA were confirmed by SPR. Biacore, as a SPR biosensor, was used for the assay, according to the user’s guide of the instrument.

＜Assay condition＞ Sensor Chip: Series S Sensor Chip CM5, Immobilization: HAS (Amine Coupling method), Buffer : 5%DMSO in PBS. ＜Result＞ “Square wave” sensorgrams were exhibited at each concentration, and concentration dependent binding of ibuprofen to HSA was confirmed. 【Method using fluorescent probes】The drug biding site of ibuprofen was confirmed using fluorescent probes which bind to drug binding site on HSA. Dansylamide (DNSA) [D5405] was used as fluorescent probe for site I, and dansylglycine (DNSG) [D5406], BD140 [D4898] were used as fluorescent probes for site II, and then bindings to site I and site II were confirmed.

＜Assay condition＞ Buffer: 1 % DMSO in phosphate buffer (pH 7.2 - 7.5); HSA: 5 µM (DNSA), 20 µM (DNSG, BD140) (50 µL/well) (Fatty acid free HSA is recommended.); Ibuprofen: each concentration (50 µL/well); DNSA: 80 µM, DNSG: 20 µM, BD140: 20 µM (50 µL/well); Incubation: 20-25 °C for 30 min; Measurement: plate-reader with excitation = 365 nm and emission = 480 nm (DNSA, DNSG), with excitation = 365 nm and emission = 585 nm (BD140). ＜Result＞ As shown in upper diagram, inhibition against binding of dansylglycine and BD140 which are fluorescent probes for site II by ibuprofen was confirmed. And also, little or extremely weak inhibition against binding of dansylamide which is fluorescent probe for site I was confirmed. In these ways, our ibuprofen can be used for study of interaction with HSA. Also, DNSA [D5405], DNSG [D5406] and BD140 [D4898] can be used for study of drug binding site on HSA.

References

Fluorescent Dye Cocktail for Multiplex Drug￢Site Mapping on Human Serum Albumin
- J. C. Er, M. Vendrell, M. K. Tang, D. Zhai, Y. T. Chang, ACS Comb. Sci. 2013, 15, 452.
High-resolution and high-throughput protocols for measuring drug/human serum albumin interactions using BIACORE
- R. L. Rich, Y. S. Day, T. A. Morton , D. G. Myszka, Anal Biochem. 2001, 296, 197.
Biosensor Analysis of the Interaction between Immobilized Human Serum Albumin and Drug Compounds for Prediction of Human Serum Albumin Binding Levels
- Å. Frostell-Karlsson, A. Remaeus, H. Roos, K. Andersson, P. Borg, M. Hämäläinen, R. Karlsson, J. Med. Chem. 2000, 43, 1986.
Characterizing a drug's primary binding site on albumin
- Y. S. Day, D. G. Myszka, J. Pharm. Sci. 2003, 92, 333.
Structural basis of the drug-binding specificity of human serum albumin
- J. Ghuman, P. A. Zunszain,I. Petitpas, A. A. Bhattacharya, M. Otagiri, S. Curry, J. Mol. Biol. 2005, 353, 38.

PubMed Literature

Articles/Brochures

Enantiomers & Racemic Mixtures

Fluorescent Probes for the Characterization of Two Specific Drug Binding Sites on HSA

Lipid Mediator Research Reagents

Anti-Rheumatoid Arthritis Agents for Experimental Use

Immune System, Inflammation, Allergy Research Reagents

Pharmaceutical Ingredients for Research and Experimental Use

TCIMAIL

[Product Highlights] Fluorescent Probe for Human Serum Albumin (HSA)-Drug Binding Assay

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Appearance	White to Almost white powder to crystal
Purity(GC)	min. 98.0 %
Purity(Neutralization titration)	min. 98.0 %
Melting point	75.0 to 78.0 °C
Solubility in Methanol	almost transparency

Melting Point	76 °C
Boiling Point	157 °C/4 mmHg
Solubility in water	Insoluble
Degree of solubility in water	21 mg/l 25 °C

Pictogram
Signal Word	Warning
Hazard Statements	H302 : Harmful if swallowed. H361 : Suspected of damaging fertility or the unborn child.
Precautionary Statements	P501 : Dispose of contents/ container to an approved waste disposal plant. P270 : Do not eat, drink or smoke when using this product. P202 : Do not handle until all safety precautions have been read and understood. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P301 + P312 + P330 : IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth. P405 : Store locked up.

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CAS RN: 15687-27-1 | Product Number: I0415

Ibuprofen

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CAS RN: 15687-27-1 | Product Number: I0415

Ibuprofen

References

References

Articles/Brochures

Safety Data Sheet (SDS)

Specifications

C of A & Other Certificates

Sample C of A

Analytical Charts

Other Documents

Product Categories

Life Science

Life Science