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CAS RN: | Product Number: B5834
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 25ML |
$50.00
|
1 | 1 | ≥80 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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Supplemental Product Information:
Product Information
Volume : 25 mLDescription
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.
Storage
Store at room temperature.
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M1948 2-Mercaptoethanol [for Electrophoresis]
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B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
| Product Number | B5834 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Green to Dark green to Dark blue clear liquid |
| Functionality test | Electrophoresis to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
Reference
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4
Articles/Brochures
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