Chemiluminescence Reagent for the Detection of Intramitochondrial Superoxide

10,10'-Dimethyl-9,9'-biacridinium bis(monomethyl Terephthalate) (MMT), developed by Kobayashi et al., is a lucigenin derivative, which is a specific probe having green chemiluminescence to superoxide among reactive oxygen species. Since amphiphilic MMT which is less hydrophilic than lucigenin possesses cell-permeability, MMT has been applicable for the detection of intramitochondrial superoxide production. Figure 1 shows the localization of MMT in mitochondria in mouse peritoneal neutrophils.