Thiol Quantification Kit

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纯度/分析方法:

別名

• Sulfhydryl Quantification Kit

文件：

SDS | 產品規格 | 檢驗報告(CoA)及其他文檔 | 分析圖譜

基本信息 | 質量規格/物性 | 安全和法規 | 應用實例・文獻

補充產品訊息

包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
1KIT	NT$21,840	15	5	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information

Description

This kit consists of three components; 5,5'-Dithiobis(2-nitrobenzoic Acid) (DTNB, Ellman’s reagent), N-Acetyl-L-cysteine (NAC), and Diluent Buffer. Reaction of DTNB with SH groups causes the solution containing them to turn yellow in color. Use of an N-Acetyl-L-cysteine standard curve allows for the quantification of SH group concentration via absorbance measurement at 412 nm.

Storage

Store at 4 °C. Use within 6 months.

Direction for Use

1. Prepare an SH group-containing sample, using Diluent Buffer to increase the volume to 200 µL if necessary.
2. Add 1 mL of Diluent Buffer to 1 vial of N-Acetyl-L-cysteine to prepare a 10 mM NAC standard solution. Dissolve carefully by vortexing.
3. Preparation of an NAC dilution series:
   Dilute the 10 mM NAC standard solution prepared in step 2 10-fold with Diluent Buffer to make a 1000 µM NAC solution (of greater than 400 µL); further dilute this solution two-fold six additional times using Diluent Buffer such that you obtain seven samples (each greater than 200 µL in volume). The dilution series comprises these seven samples, along with a blank containing only Diluent Buffer. The concentration range this dilution series offers is appropriate in many cases, however if you plan on measuring a more dilute sample, adjust concentrations as needed.
4. Aliquot 100 µL of each sample or NAC solution to each of two wells of a 96-well plate.
5. Prepare DTNB solution by adding 1 mL of Diluent Buffer to 1 vial of DTNB. Dissolve carefully by vortexing. (Use DTNB solution as quickly as possible following preparation.)
6. Add 15 µL of this DTNB solution to each well.
7. Incubate at room temperature for 10 minutes.
8. Measure the absorbance at 412 nm. (Broad range measurement from 380 - 440 nm is also possible.)
9. Create a standard curve by plotting the absorbance of each standard solution against its SH concentration in μM. Use this standard curve to determine the SH concentration of your samples.

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