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CAS RN: | 產品號碼: T3885
Streptavidin R-PE Conjugate
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補充產品訊息:
Product Information
Volume/Quantity : 0.1 mgProduct Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE (Ex: 496 nm, Em: 575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin
Application
Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400). Western blotting (1:1000).
Storage
Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.
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| 產品號碼 | T3885 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Refrigerated (0-10°C) |
| 應避免的情況 | Light Sensitive,Heat Sensitive |
產品規格
| Appearance | Light red to Red clear liquid to cloudy liquid |
| Specific Activity | to pass test(FLISA) |
性質
GHS
相關法規
運輸資料
| HS編碼* | 3504.00-000 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Application
Flow Cytometry of Stained Cells
The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1μg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.
References
- Guidelines for the use of flow cytometry and cell sorting in immunological studies
TCIMail
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