Maximum quantity allowed is 999
请选择数量
CAS RN: | 產品號碼: T3531
Streptavidin Maleimide Conjugate
* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與
當地經銷商
洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。
補充產品訊息:
Product Information
Volume/Quantity:0.5 mg
Conjugation:Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form:Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Preservative:None
Specific Activity:>10 U/mg , HABA dye-binding method
Description
Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.
Protocol
1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 ºC or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.
Storage
Store at －20 ºC to －80 ºC (Preferably at －80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
Related Products
S0951 Streptavidin from Streptomyces avidinii
S0966 Streptavidin FITC Conjugate
S0972 Streptavidin HRP Conjugate
S0993 Streptavidin DTBTA-Eu3+ Conjugate
T3885 Streptavidin R-PE Conjugate
A2959 Anti-Endo-M Polyclonal Antibody Biotin Conjugate
A3010 Anti-6xHis Monoclonal Antibody (6A12) Biotin Conjugate
G0387 Goat Anti-Mouse IgG Biotin Conjugate
G0389 Goat Anti-Rabbit IgG Biotin Conjugate
G0432 Goat Anti-Mouse IgM Biotin Conjugate
M3053 Mouse Anti-Human IgG Fc Biotin Conjugate
S0883 Sulfo-SMCC Sodium Salt
H0586 4'-Hydroxyazobenzene-2-carboxylic Acid
| 產品號碼 | T3531 |
| 外觀與形狀（20°C） | Solid |
| 儲存條件 | Frozen (-80°C) |
| 應避免的情況 | Heat Sensitive |
| PubChem Substance ID | 354335029 |
產品規格
| Appearance | White to Brown to Dark green powder to crystal |
| Specific Activity | to pass test |
性質
GHS
相關法規
運輸資料
| HS編碼* | 3504.00-000 |
Application
Streptavidin Maleimide Conjugate
References
- Spectrophotometric determination of avidin and biotin.
- Isolation and characterization of highly purified streptavidin obtained in a two-step purification procedure from Streptomyces avidinii grown in a synthetic medium.
- G. T. Hermanson, in Bioconjugate Techniques, 2nd ed., Academic Press, 2008, pp.905-909.
文章/手冊
產品文件 （部分產品的分析圖譜無法提供，敬請諒解。）
SDS
請選擇語言。
請求的SDS不可用。
如需更多幫助，請聯繫我們 。
產品規格
檢驗報告(CoA)及其他文檔
請輸入批號
輸入的批號不正確
示例 CoA
可下載CoA示例。注：該示例可能非最新批次的CoA。
目前沒有該產品的 CoA 示例。
分析圖譜
請輸入批號
輸入的批號不正確
很抱歉，您搜索的分析圖譜無法提供。