Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

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補充產品訊息

包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
1KIT	NT$6,200	24	0	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent for the chemiluminescent detection of HRP-labeled probes that react with proteins transferred to the membrane by Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. It allows the detection of proteins at the nanogram level.

Storage

• Store at 4 °C, protected from light. Use within 6 months.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a blotting membrane covered with HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane with the protein blotted side up on the plastic wrap or polyethylene sheet.
  6. Pour the mixture over the surface of the membrane.
  7. Incubate the membrane with this product for 60 seconds at room temperature.
  8. Remove excess mixture using paper.
  9. Place the membrane in a new wrap or polyethylene sheet.
  10. Image the membrane using an imaging system or X-ray film.

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