Biotin-PEG2-NHS (2mg×5)

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纯度/分析方法:

別名

• 9-(Biotinamido)-4,7-dioxanonanoic Acid N-Succinimidyl Ester (2mg×5)
• N-Succinimidyl 9-(Biotinamido)-4,7-dioxanonanoate (2mg×5)

文件：

SDS | 產品規格 | 檢驗報告(CoA)及其他文檔 | 分析圖譜

基本信息 | 質量規格/物性 | 安全和法規 | 應用實例・文獻

補充產品訊息

包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
1SET	NT$5,600	1	5	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information
Volume/Quantity : 2 mg x 5
Molecular weight : 500.6

Description
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6097, Biotin-PEG₂-NHS reacts with the primary amines (-NH₂) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 40 mg of antibodies.

Storage
This product should be stored at -20 ºC. Do not store at stock solution.

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Directions for Use
Preparation:
Use of a 10 mM Biotin-PEG₂-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-PEG₂-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.

Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10^-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6097 solution] x 10^-6 [L/uL] x 10 [mmol/L] x 10^-3 [mol/mmol]

A = 20 [uL of 10 mM B6097 solution]

Procedure:
1. Bring Biotin-PEG₂-NHS (Product No. B6097) to room temperature.
2. Dissolve 2 mg of Biotin-PEG₂-NHS (Product No. B6097) in 400uL of PBS and prepare a 10 mM solution of Biotin-PEG₂-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-PEG₂-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.