endo-α-N-Acetylgalactosaminidase (=Endo-α) Recombinant: from Bifidobacterium longum expressed in Escherichia coli

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別名

• Endo-α Recombinant: from Bifidobacterium longum expressed in Escherichia coli

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補充產品訊息

包裝	價格	同一天	2-3个工作日
1VIAL	NT$6,960	13	聯繫我們

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大包裝詢價

Product Information

• Quantity : 100 mU per vial
• Product Form : Purified Endo-α, Lyophilized powder
• Preservative : None
• Stabilizer : None

Description

• This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.

Unit Definition

• One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No. G0375) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.

Storage

• Store at -80°C in its lyophilized powder form. Use promptly after dissolving in buffer.

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