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CAS RN: 9001-67-6 | 產品號碼: I1205

Sialidase from Arthrobacter ureafaciens


纯度/分析方法:
別名
  • Neuraminidase from Arthrobacter ureafaciens
  • α2-3,6,8 Sialidase from Arthrobacter ureafaciens
文件:
1VIAL
NT$5,480
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補充產品訊息:

Product Information

  • Quantity : 1.0 U per vial (10 U/mL)
  • Product Form : Purified Sialidase, Liquid
  • Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
  • Preservative : None
  • Stabilizer : None

Description

  • This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

  • One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

  • Reaction conditions: 50 mM Sodium acetate, pH 5.0
    The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

  • Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
    (For long-term use, aliquot and freeze after thawing.)

Related Products

  • G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
  • I1206 Sialidase (Neuraminidase) Agarose

  • Other oligosaccharide-releasing enzymes
  • [Endo-type N-glycanase (ENGase)]
  • A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
  • E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
  • G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)

  • [Endo-type O-glycanase]
  • A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)

  • [Endo-type glycoceramidase]
  • R0240 rEGCase I
  • R0241 rEGCase I assisted by Activator II
  • R0242 rEGCase II
  • R0243 rEGCase II assisted by Activator II

  • [Endo-type glycosaminoglycanase]
  • K0069 Keratanase II (for hydrolysis of Keratan sulfate)

產品號碼 I1205
外觀與形狀(20°C) Liquid
儲存條件 Frozen (-20°C)
應避免的情況 Heat Sensitive
CAS RN 9001-67-6
產品規格
Appearance Colorless to Almost colorlessclear liquid
Titer min. 1.0 unit/vial
Molecular weight 88, 66, 52 kDa
性質
GHS
相關法規
運輸資料
HS編碼* 3507.90-000
*此H.S.編碼用於日本出口報關, 不適用於您所在國家或地區的進口申報
產品文件 (部分產品的分析圖譜無法提供,敬請諒解。)
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產品規格
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