CAS RN: | Product Number: T3940

Tissue-Clearing Reagent iTOMEI-D [for Plants]

Purity:

Synonyms:

Product Documents:

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
5ML	NT$1,218	3	0	Contact Us	Shipping Simulation
25ML	NT$3,696	20	0	Contact Us	Shipping Simulation

* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact our distributor in Taiwan to order our product.
* The storage conditions are subject to change without notice.

Supplemental Product Information:

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents;

Paraformaldehyde (PFA)
PBS
Stains* (option)
Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr
PBS Wash ×3
Decoloring
Reagent : iTOMEI-D (TCI Product No. T3940)
Time : 24hr
PBS Wash ×3
(Optional process Staining*, PBS Wash ×3)
Clearing (RI matching)
Reagent : Mounting solution**
Time : Operation time is determined by the mounting solution.
Mounting
Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.

Related Products

P0018　Paraformaldehyde
A2412　DAPI·2HCl
E0197　Evans Blue
A0132　Acridine Orange
A0321　Tris-Base
G0316　Glycerol [for Electrophoresis]
S0111　D-(+)-Sucrose
T3530　Tissue-Clearing Reagent TOMEI [for Plants]

General Information plus

Specifications & Properties plus

Specifications

Appearance	Colorless to Almost colorless clear liquid
Functionality test(fluorescence retention)	to pass test
Functionality test(Tissue bleaching)	to pass test

Properties (reference)

Safety & Regulations plus

GHS

Pictogram
Signal Word	Warning
Hazard Statements	H315 : Causes skin irritation. H319 : Causes serious eye irritation.
Precautionary Statements	P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention.