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CAS RN: 23491-45-4 | Product Number: B6236
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
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Purity:
Synonyms:
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride (0.2mL×5) (1mg/mL in Water)
- 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-[2,5'-bibenzimidazol]-2'-yl]phenol Trihydrochloride (0.2mL×5) (1mg/mL in Water)
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
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Supplemental Product Information:
Product Information
Volume : 0.2 mL x 5 vial
Concentration : 1.0 mg/mL
Description
Bisbenzimide H 33258 is a fluorescent stain for labeling DNA.
DNA-bound Bisbenzimide H 33258 is readily excited with UV and emits fluorescence in the blue region of the visible light spectrum (Excitation : 355 nm / Emission : 465 nm).
Description for Use
Fluorescence Staining of Cell Nucleus
Fix and permeabilize cells as desired.
Dilute Bisbenzimide H 33258 solution 1/1000* with PBS or an appropriate buffer.
Incubate with cells at room temperature for 5-20 minutes**.
*Typical working concentration used in most applications is 0.1μg/mL-10μg/mL.
Incubate with cells at room temperature for 5-30 minutes**.
** Extend Incubate time as desired.
Mount sample with an appropriate medium and proceed to imaging.
Storage
Store at －20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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| Product Number | B6236 |
| Molecular Formula / Molecular Weight | C__2__5H__2__4N__6O·3HCl = 533.88 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 23491-45-4 |
| Reaxys Registry Number | 4088183 |
| PubChem Substance ID | 468590991 |
| MDL Number | MFCD00012679 |
Specifications
| Appearance | Light yellow to Yellow clear liquid |
| DNA staining test | pass test |
Properties (reference)
| Boiling Point | 100 °C |
GHS
Related Laws:
| RTECS# | SM1140500 |
Transport Information:
| H.S.code* | 2933.59-000 |
Articles/Brochures
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Specifications
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Other Documents
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