WST-8 Reagent [for Cell Proliferation Assay]

×

Purity:

Synonyms:

Product Documents:

SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications & Literature

Supplemental Product Information

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
1ML	$46.00	25	11	Contact Us	Shipping Simulation

* Please contact our distributors or TCI to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.

Request Bulk Quote

Product Information

• Volume : 1 mL (100 rxn)

Description

• This product is supplied as a ready-to-use reagent for the measurement of relative cell number. Reduction of the water-soluble tetrazolium salt WST-8 by the cell membrane-permeable electron carrier 1-MePMS converts it to a highly colored form which can be detected using a plate reader fitted with a 460 nm absorbance filter. As 1-MePMS is itself reduced by intracellular NADH/NADPH, and NADH/NADPH concentration is relatively constant over the course of the cell cycle, the amount of reduced WST-8 and therefore the degree of color change can be used to determine relative cell number,

Direction for Use

• 1. Prepare cell samples in a 96 well plate (do not remove medium).
  2. Apply 10 µL of W0023 to each well and incubate for up to 4 hours* at 37°C / 5% CO₂.
  3. Measure the absorbance at 460 nm.
• *Optimal incubation times should be determined for each individual cell line

Storage

• Store at 4°C. Use within 6 months of receipt.