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用于快速且高灵敏度检测蛋白质的凝胶负染色试剂盒
检测电泳后聚丙烯酰胺凝胶中的蛋白质谱带需要对凝胶进行染色。常用的染色方法包括考马斯亮蓝染色、银染色、荧光染色和负染色。负染色是一种不会染色含蛋白质的凝胶区域,而其余部分被染成白色的染色方法1)。通过将凝胶置于深色背景下,可以观察到谱带。
凝胶负染色试剂盒 (1)可在短短20分钟内高灵敏度地检测出蛋白质谱带。此外,使用附带的脱色液,可以轻松地将凝胶脱色,脱色的凝胶可进一步用于下游,如蛋白质印迹和质谱2,3)。
图1. 使用1染色后,不同浓度下含分子量标记的凝胶
图2. A: 用1染色的负载小鼠血清的凝胶 B: 将图2A中的凝胶脱色,并通过蛋白质印迹检测小鼠IgG
关键词
- G0615
- 凝胶负染色试剂盒 [电泳用]
- A3217
- 30%丙烯酰胺/双丙烯酰胺 (29:1) [电泳用]
- A3218
- 30%丙烯酰胺/双丙烯酰胺 (37.5:1) [电泳用]
- B5834
- 2X SDS-PAGE样品缓冲液 (无2-巯基乙醇) [电泳用]
- B6104
- 4X SDS-PAGE样品缓冲液 (不含2-巯基乙醇) [电泳用]
- B6105
- 6X 样品缓冲液 (不含2-巯基乙醇) [电泳用]
- B6110
- 2X SDS-PAGE样品缓冲液酚红 (不含2-巯基乙醇) [电泳用]
- C3488
- 考马斯亮蓝G-250 (即用型溶液) [电泳用]
文献
- 1) Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
- 2) A procedure for protein elution from reverse-stained polyarcylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
- 3) High Yield Elution of Proteins from Sodium Dodecyl Sulfate–Polyacrylamide Gels at the Low-Picomole Level. Application to N-Terminal Sequencing of a Scarce Protein and to In-Solution Biological Activity Analysis of On-Gel Renatured Proteins