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用于线粒体内超氧化物检测的化学发光试剂
Kobayashi等发展了一种光泽精衍生物10,10'-Dimethyl-9,9'-biacridinium Bis(monomethyl Terephthalate) (MMT),这是一种特殊的探针,在活性氧中对超氧化物有绿色荧光。两亲性MMT的亲水性比光泽精弱,它具有细胞渗透性,因此可用于检测线粒体内超氧化物的产生。图1展示了老鼠腹膜中性粒细胞的线粒体中MMT的定位。
文献
- Specific detection of intramitochondrial superoxide produced by either cell activation or apoptosis by employing a newly developed cell-permeative lucigenin derivative, 10,10'-dimethyl-9,9'-biacridinium bis(monomethyl terephthalate)