Maximum quantity allowed is 999
CAS RN: 62229-50-9 | 产品编码: R0262
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Description
This product contains 100 µg of recombinant human EGF produced in E. Coli, lyophilized from a 1mg/mL solution containing 1x PBS. Recombinant human EGF comprises a 53 amino acid-length, non-glycosylated polypeptide chain, with a molecular mass of 6.2kDa. Apart from being a potent mitogenic factor, EGF stimulates the proliferation of a wide variety of epithelial cells, and is a common medium additive in both 2D and 3D cell culture.
Specifications
Biological Activity (Balb/c Proliferation Assay) : ED50 < 0.1 ng/mL (> 1 x 107 IU/mL)Purity (SDS/PAGE) : >95.0%
Mycoplasma Contamination (PCR) : No bands corresponding to Mycoplasma detected
Endotoxin Level (Chromogenic LAL Assay) : < 1.0 EU/µg
Amino Acid Sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Storage
Store at -80°C. Post-reconstitution, the addition of a carrier protein such as 0.1% BSA is recommended for improved stability. Avoid multiple freeze-thaw cycles.
产品编码 | R0262 |
外观与形状(20°C) | 固体 |
储存温度 | 冷冻 (-80°C) |
应避免的情况 | 加热 |
CAS RN | 62229-50-9 |
Appearance | White to Almost white powder to crystal |
Biological Activity (Balb/c-3T3 proliferation assay) | ED50 ≤ 0.1ng/mL |
Endotoxin LevelEndotoxin Level (chromogenic LAL assay) | max. 1.0 EU/µg |
Mycoplasma Contamination (PCR) | No bands corresponding to mycoplasma were detected |
Purity (SDS-PAGE/CBB) | min. 95.0 % |
HS编码* | 3504.00-000 |
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