rEGCase I assisted by Activator II

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纯度/分析方法:

别名：

• 激活剂II辅助型rEGCase I
• 激活剂II辅助型重组神经节苷脂内切糖苷酶I
• Recombinant Endoglycoceramidase I assisted by Activator II
• Recombinant - Rhodococcus equi EGCase I expressed in Rhodococcus erythropolis
• Recombinant Endoglycoceramidase I with Activator II

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补充产品信息

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1VIAL	S$553.50	6	1	请联系我们		货期预估

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大包装询价

Product Information

• Quantity : 300 mU per vial (3 mU/μL) and 50 nmol Activator II per vial (0.5 nmol/μL)
• Product Form : Purified recombinant EGCase I and Activator II, Liquid
• Storage buffer : 20 mM Sodium phosphate, 50 mM NaCl buffer, pH 7.0
• Preservative : None
• Stabilizer : None

Description

• Endoglycoceramidase I (EGCase I) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under a detergent-containing condition. On the other hand, Activator II is capable of inducing activity of EGCase without any detergent reagents. This product is provided as a mixture-form of EGCase I and Activator II. By using this product, cell-surface glycosphingolipids of living cells are hydrolyzed without cell disruption caused by addition of detergent. The EGCase I gene is derived from Rhodococcus equi and its recombinant protein is expressed in Rhodococcus erythropolis.

Unit Definition

• One unit (U) of EGCase I is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.

Assay Conditions

• Reaction conditions: 50 mM sodium acetate, pH 5.5
  The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL (without detergent). The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference).
• [Reference]
  Determination of Glucose by a Modification of Somogyi-Nelson Method
  C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.
• 
  
• Regarding hydrolysis of cellular GSLs, see the reference (Methods Mol. Biol.) below.
• [Reference]
  Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi
  M. Ito, Y. Ishibashi, T. Watanabe, J. Iwaki, T. Kurita, N. Okino, Methods Mol. Biol. 2023, 2613, 229.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.

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