rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]

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纯度/分析方法:

别名：

• rSRL-生物素 [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
• rSRL-生物素结合物
• 重组齐整小核菌凝集素(= rSRL)-生物素结合物
• rSRL-Biotin Conjugate
• Recombinant Sclerotium rolfsii lectin (= rSRL)-Biotin Conjugate

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规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1ML	S$478.50	10	2	请联系我们		货期预估

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Product Information
Product code: R0233
Product name: rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >16 kDa
Preservative : None
Stabilizer: None

Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rSRL-Biotin shows significant interaction with agalacto human transferrin (hTF) possessing [GlcNAcβ(1-2)Man] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rSRL-Biotin specifically binds to β(1-2)GlcNAc-terminated hTF but not to non-β(1-2)GlcNAc-terminated glycoproteins such as hTF, asialo hTF and bovine RNase B.

Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

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