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CAS RN: | 产品编码: R0232
rMOA-Biotin [for Galα(1-3)Gal]
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纯度/分析方法:
别名：
- rMOA-生物素 [for Galα(1-3)Gal]
- rMOA-生物素结合物
- 重组硬柄小皮伞凝集素(= rMOA)-生物素结合物
- rMOA-Biotin Conjugate
- Recombinant Marasmius oreades Agglutinin (= rMOA)-Biotin Conjugate
- Recombinant Marasmius oreades lectin (= rMOA)-Biotin Conjugate
产品文档：
补充产品信息:
Product Information
Product code: R0232
Product name: rMOA-Biotin [for Galα(1-3)Gal]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant MOA, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >32 kDa
Preservative : None
Stabilizer: None
Description
The rMOA is a carbohydrate-binding protein (Lectin) derived from Marasmius oreades. The rMOA gene product is expressed in Escherichia coli. This lectin preferably binds to a [Galα(1-3)Gal] glycan epitope.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rMOA-Biotin shows significant interaction with Cetuximab possessing [Galα(1-3)Gal] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rMOA-Biotin specifically binds to α(1-3)Gal-terminated Cetuximab but not to non-α(1-3)galactosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
| 产品编码 | R0232 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 冷冻 (-20°C) |
| 应避免的情况 | 加热 |
技术规格
| Appearance | Colorless to Almost colorless clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 25 to 40 kDa |
| Suitability for use in ELISA tests | min. 1.0 (1.0 micro g/mL, OD450) |
物性（参考值）
GHS
相关法规
运输信息
| HS编码* | 3504.00-000 |
应用
Recombinant lectin
References
- Comparison of the binding properties of the mushroom Marasmius oreades lectin and Griffonia simplicifolia I-B4 isolectin to αgalactosyl carbohydrate antigens in the surface phase
- Cloning, expression, and characterization of the Galα1,3Gal high affinity lectin from the mushroom Marasmius oreades
- Structural characterization of a lectin from the mushroom Marasmius oreades in complex with the blood group B trisaccharide and calcium
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