Maintenance Notice (10:30 PM January 4 - 8:00 AM January 5, 2025 UTC): This website is scheduled to be unavailable due to maintenance. We appreciate your patience and understanding.
Published TCIMAIL newest issue No.197 | Notice of Discontinuing the Use of Password-Protected Compressed Files | Various analytical charts can be searched on each product detail page and Product Document Search (The kinds of analytical charts differ by product)
Maximum quantity allowed is 999
请选择数量
CAS RN: | 产品编码: N1113
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_pdp.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_mobile.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_thumbnail.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
×
![Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]")
补充产品信息:
Product Information
Volume : 5 mL
Description
This product is supplied as a concentrated solution containing Nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.
Direction for Use
1: Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
2: Dilute NBT/ X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl)
3: Incubate the washed membrane with 1X NBT/ X-Phosphate p-Toluidine Salt solution until color development.
4: Add deionized water to stop the color development.
Storage
The product should be stored at 4 ºC. Don’t freeze because of arising precipitate.
Related Products
B1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro blue tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
| 产品编码 | N1113 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 冷藏 (0-10°C) |
技术规格
| Appearance | Yellow to Amber to Dark purple clear liquid |
| Alkaline Phosphatase detection test | to pass test |
物性（参考值）
GHS
| 象形图 |
|
| 信号词 | Warning |
| 危险性说明 | H373 : May cause damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
| 防范说明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P314 : Get medical advice/ attention if you feel unwell. P403 : Store in a well-ventilated place. |
相关法规
运输信息
| HS编码* | 3822.19-000 |
应用
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p407, p505, p598
TCIMail
产品文档 (部分产品的分析图谱无法提供，敬请谅解。)
化学品安全说明书(SDS)
请选择语言。
如需更多帮助，请联系我 们。
技术规格
CoA及其他文档
请输入批号
批号输入有误。请输入中横线前的4-5个字母数字字符。
示例 CoA
可下载CoA示例。注：该示例不一定是最新批次的CoA。
目前没有该产品的 CoA 示例。
分析图谱
请输入批号
批号输入有误。请输入中横线前的4-5个字母数字字符。
很抱歉，您搜索的分析图谱无法提供。