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CAS RN: 23491-45-4 | 产品编码: H1343
Bisbenzimide H 33258 [for Biochemical Research]
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![Bisbenzimide H 33258 [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "Bisbenzimide H 33258 [for Biochemical Research]")
| 规格 | 单价 | 同一天 | 2-3个工作日 | 左侧以上货期 | 数量 |
货期信息
|
|---|---|---|---|---|---|---|
| 25MG |
S$82.50
|
10 | 5 | 下单后约3-4周内可以发货 |
|
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| 产品编码 | H1343 |
| 纯度/分析方法 | >98.0%(HPLC) |
| 分子式/分子量 | C__2__5H__2__4N__6O·3HCl = 533.88 |
| 外观与形状（20°C） | 固体 |
| 储存温度 | 冷冻 (<0°C) |
| 应避免的情况 | 光,加热 |
| 包装和容器 | 25MG-Glass Bottle with Plastic Insert （查看图片） |
| CAS RN | 23491-45-4 |
| 相关CAS RN | 2138819-37-9 |
| Reaxys-RN | 4088183 |
| PubChem物质ID | 172089156 |
| MDL编号 | MFCD00012679 |
技术规格
| Appearance | Light yellow to Brown to Dark green powder to crystal |
| Purity(HPLC) | min. 98.0 area% |
| Water(Vaporization method) | max. 20.0 % |
| DNA staining test | to pass test |
物性（参考值）
| 熔点 | 280 °C |
GHS
| 象形图 |
|
| 信号词 | Warning |
| 危险性说明 | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| 防范说明 | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
相关法规
| RTECS# | SM1140500 |
运输信息
| HS编码* | 2933.59-000 |
应用
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
应用
Fluorometric Assays of DNA Using Bisbenzimide H 33258
References
- A simple, rapid, and sensitive DNA assay procedure
- Fluorometric assay of DNA in cartilage explants using Hoechst 33258
- Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye
- DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
- Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies
- Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study
参考文献
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其他文件
相关产品
![Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/B6236.jpg?context=bWFzdGVyfHJvb3R8MTc2MTF8aW1hZ2UvanBlZ3xhREE1TDJnNU15ODVNVFF3T0RjNE1URTBPRFEyTDBJMk1qTTJMbXB3Wnd8MWEwZWI2OGMxZjUwNmMzYzU2ODU5MjA2MjNkZjk5MTliMGM3MGQ2MmVhNjFjN2I1ZDcxNTcwODcwZTBhNGFmYw "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
| 产品 | B6236 |
| CAS RN | 23491-45-4 |
| 纯度/分析方法 |