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CAS RN: 41085-99-8 | 产品编码: D6202
DiI Solution [for Cell Membrane Staining]
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Product Information
- Concentration :1 mM
- Excitation / Emission : 553 nm / 575 nm
Description
- DiI is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiI which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature (below 15 °C is recommended) and protect from light.
Directions for Use: Staining live cells
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- Dilute DiI solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiI-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
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- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiI solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiI-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Related Products
- D6263 DiD Solution [for Cell Membrane Staining]
| 产品编码 | D6202 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 室温 (15°C以下阴凉干燥处) |
| 应避免的情况 | 光 |
| CAS RN | 41085-99-8 |
技术规格
| Appearance | Light red to Red clear liquid |
| Cell staining test | to pass test |
物性（参考值）
| 比重 | 1.04 |
GHS
| 象形图 |
|
| 信号词 | Danger |
| 危险性说明 | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H340 : May cause genetic defects. H335 : May cause respiratory irritation. H336 : May cause drowsiness or dizziness. H402 : Harmful to aquatic life. H225 : Highly flammable liquid and vapor. |
| 防范说明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P273 : Avoid release to the environment. P270 : Do not eat, drink or smoke when using this product. P202 : Do not handle until all safety precautions have been read and understood. P240 : Ground and bond container and receiving equipment. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233 : Keep container tightly closed. P201 : Obtain special instructions before use. P243 : Take action to prevent static discharges. P241 : Use explosion-proof electrical/ ventilating/ lighting/ equipment. P242 : Use non-sparking tools. P271 : Use only outdoors or in a well-ventilated area. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P303 + P361 + P353 : IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P304 + P340 + P312 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor if you feel unwell. P403 + P233 : Store in a well-ventilated place. Keep container tightly closed. P403 + P235 : Store in a well-ventilated place. Keep cool. P405 : Store locked up. |
相关法规
运输信息
| UN编号 | UN1170 |
| 类别 | 3 |
| 包装类别 | II |
| HS编码* | 3822.19-000 |
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