4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

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纯度/分析方法:

别名：

• 4X SDS-PAGE样品缓冲液 ( 不含2-巯基乙醇) [电泳用]

产品文档：

SDS | 技术规格 | CoA及其他文档 | 分析图谱

基本信息 | 技术规格和文件 | 安全和法规 | 应用实例・文献

补充产品信息

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
20ML	S$75.00	≥100	8	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

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大包装询价

Product Information

• Volume : 20 mL

Description

• This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
  SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
  Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

• Preparation of non-reducing sample
  A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
• Preparation of reducing sample (reducing with 2-Mercaptoethanol)
  Add 20 μL of 2-Mercaptoethanol per 80 μL of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95–100°C for 5 minutes. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
  *Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

• Store at room temperature.

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