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CAS RN: | 产品编码: B5834
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Product Information
Volume : 25 mLDescription
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.
Storage
Store at room temperature.
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| 产品编码 | B5834 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 室温 (15°C以下阴凉干燥处) |
技术规格
| Appearance | Green to Dark green to Dark blue clear liquid |
| Functionality test | Electrophoresis to pass test |
物性（参考值）
GHS
相关法规
运输信息
| HS编码* | 3822.19-000 |
应用
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
Reference
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4
TCIMail
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