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CAS RN: | Product Number: P2461
Protein A Agarose
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Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time | Quantity |
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|---|---|---|---|---|---|---|
| 1VIAL |
S$382.50
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8 | 0 | Contact Us |
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Product Information
Volume/Quantity : 2 mL
Product Form : Protein A agarose is supplied pre-swollen in 20% ethanol.
Preparation : Recombinant Protein A is covalently coupled to agarose gel.
Preservative : 20% ethanol
Description
Protein A is a type I membrane protein produced by several strains of Staphylococcus aureus. It has high-affinity binding sites that enable the purification of IgG obtained from various species such as humans, rabbit, mouse, and bovine. Protein A Agarose is prepared using a covalent coupling method that maintains the stability and binding characteristics of the product.
By using our resin, human IgGs can be eluted under milder conditions (such as at pH 4.0) as compared to elution by conventional methods.
Additional information is available on our website.
Applications
Protein A agarose can be used antibody purification.
Storage
Protein A agarose should be stored in neutral pH at 2-8 ºC in 20% ethanol or other bacteriostatic agent, e.g. sodium azide. The resin must not be frozen.
Related Products
P2366 Protein A Recombinant, expressed in Escherichia coli
P2407 Protein A Biotin Conjugate
P2466 Protein A HRP Conjugate
| Product Number | P2461 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
| PubChem Substance ID | 354334260 |
| MDL Number | MFCD00165563 |
Specifications
| Appearance | White to Light gray to Light yellow clear liquid to cloudy liquid |
| Binding capacity | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3504.00-000 |
Application
Protein A Agarose for the Elution of Antibodies under Mild Conditions
Fig. 1. Purification of human IgG using TCI’s protein A agarose. Most antibodies are eluted at pH 4.0. 
Fig. 2. Purification of human IgG using competitor’s product.
References
- Use of staphylococcal protein A as an immunological reagent
- Staphylococcal protein A consists of five IgG-binding domains
Articles/Brochures
TCIMAIL
[TCIMAIL No.174] Protein A Agarose for Antibody Purification[Product Highlights] Protein A Agarose for the Elution of Antibodies under Mild Conditions
[Featured Products] Antibody-Binding Proteins: Protein A, Protein G, Protein L
Product Documents (Note: Some products will not have analytical charts available.)
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Specifications
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