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CAS RN: | Product Number: N1113
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
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Purity:
Synonyms:
- NBT / X-Phosphate p-Toluidine Salt Solution (50X)
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 5ML |
$69.00
|
10 | 0 | Contact Us |
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* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
Volume : 5 mL
Description
This product is supplied as a concentrated solution containing Nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.
Direction for Use
1: Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
2: Dilute NBT/ X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl)
3: Incubate the washed membrane with 1X NBT/ X-Phosphate p-Toluidine Salt solution until color development.
4: Add deionized water to stop the color development.
Storage
The product should be stored at 4 ºC. Don’t freeze because of arising precipitate.
Related Products
B1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro blue tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
| Product Number | N1113 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
Specifications
| Appearance | Yellow to Amber to Dark purple clear liquid |
| Alkaline Phosphatase detection test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H373 : May cause damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P314 : Get medical advice/ attention if you feel unwell. P403 : Store in a well-ventilated place. |
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p407, p505, p598
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