Dansyl Hydrazine [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, and can easily react with a carbonyl group to form the corresponding hydrazone. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 340 nm and 525 nm, respectively.

Application examples:
[Ketosteroids] ^1~4)
A dried sample, 0.2 mL of an alcoholic hydrochloric acid (conc. HCl 0.65 mL / ethanol 1 L), and 0.2 mL of the labeling reagent 1 in alcohol (2 mg / mL) are mixed, and heated on a water bath for 10 min. 0.2 mL of alcohol containing sodium pyruvate (5 mg / mL) is added to decompose the excess labeling reagent. The reaction mixture is allowed to stand at room temperature for 15 min, ether (6 mL) and 0.5 N NaOH (3 mL) are added and shaken. After an extraction procedure, the solvent is evaporated, chloroform (0.2~0.5 mL) is added to the residue, and use as the HPLC sample.
[Others]
Hydrocortisone in body fluid^3,4), reducing sugars, steroids in serum and urine⁵⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )