text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Gelieve het aantal te selecteren

Antibody Stripping Solution for Chemiluminescent Western Blotting

Western blot stripping buffer (1) is a reagent to remove antibodies from membranes after Western blotting detection using chemiluminescence.1,2) Depending on the affinity of antigen and antibody, the antibody can be removed from the membrane by soaking the membrane in 1 after detection and allowing it to react for 30 minutes at room temperature. Because antigens on the membrane are retained after removal of antibodies, detection can be performed again using another primary antibody. In addition, the advantage is that multiple antigens can be detected in a single electrophoresis and transfer, saving sample and experimental time.

Figure. Two-fold dilution series of HeLa cell lysate were separated by SDS-PAGE (5 - 0.078 µg/lane).
  • (A) First detection. Detected by binding anti-αTubulin antibody (Rabbit IgG).
  • (B) Antibodies were stripped using stripping buffer from 1or other company product.
  • (C) Second detection. The stripped membrane was blocked again and detected by binding with anti-GAPDH antibody (Mouse IgG).

Related Products

W0024
Western Blot Stripping Buffer [for Biochemical Research]
A3217
30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218
30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B5834
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104
4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110
2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]

Related Product Category Pages

References

Sessiestatus
Uw sessie verloopt over 10 minuten. Na het verlopen van de sessie word u doorgestuurd naar de startpagina. Klik op de knop om de sessie vanaf dezelfde pagina voort te zetten. minuut. Na het verlopen van de sessie word u doorgestuurd naar de startpagina. Klik op de knop om de sessie vanaf dezelfde pagina voort te zetten.

Uw sessie is verlopen. U wordt doorgestuurd naar de startpagina.