Nuclear / Cytoplasmic Fractionation Kit

×

Zuiverheid:

Synoniemen

Productdocumenten:

SDS | Specificatie | Analyse certificaat & andere certificaten | Analytische grafieken

Algemene informatie | Specificaties en eigenschappen | Veiligheid en regelgeving | Toepassing & Literatuur

Aanvullende productinformatie

Toelichting bij deze pagina

Afmeting	Eenheidsprijs	België	Japan*	Hoeveelheid	Verzendinformatie
1KIT	€200.00	1	6		Simulatie van verzending

*Voorraad beschikbaar in België: Verzending dezelfde dag
*Voorraad beschikbaar in Japan: Raadpleeg de verzendsimulatie voor geschatte levertijden. (met uitzondering van gereguleerde producten en zendingen met droogijs)

Toegevoegd aan uw winkelwagen

Voeg toe aan winkelmandje

Bulkofferte aanvragen

Product Information

• Components :1×Cytoplasmic Extraction Buffer, 20×Detergent solution, 1×Nuclear Extraction Buffer
• Size : 50 tests

Description

• This product is supplied for fractionating nuclear proteins and cytoplasmic proteins easily from cultured mammalian cells. Extracted protein can be used in downstream applications such as Western blotting.

Directions for Use

• [For Cytoplasmic Extraction]
  • 1. Collect cells and wash twice with cold PBS. Use a cell scraper for adherent cells.
  • 2. Resuspend the cells gently in cold 1×Cytoplasmic Extraction Buffer. Add 400 μL of the buffer to 100 μL of the cell volume (~ 1 × 10⁷ cells).
  • 3. Incubate on ice for 10 minutes.
  • 4. Add 20×Detergent solution to the cell suspension and vortex vigorously for 10 seconds. Use 25 μL of the solution per 500 μL of the mixture.
  • 5. Centrifuge at 800 × g for 10 minutes at 4 °C.
  • 6. Transfer the supernatant (cytoplasmic fraction) to a new tube.
  [For Nuclear Extraction]
  • 7. Resuspend the pellet in cold 1×Nuclear Extraction Buffer. Add 100 μL of the buffer to 100 μL of the pellet volume.
  • 8. Incubate on ice for 20 minutes, vortexing every 5 minutes.
  • 9. Centrifuge at 15,000 × g for 10 minutes at 4 °C.
  • 10. Transfer the supernatant (nuclear fraction) to a new tube.

Storage

• Store at 4 °C.

Related Products

• P2949 100×Protease Inhibitor Cocktail [EDTA free]
• P2976 100×Protease Inhibitor Cocktail