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CAS RN: | Product Number: W0023
WST-8 Reagent [for Cell Proliferation Assay]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1ML |
$51.00
|
2 | Contact Us | 37 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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Supplemental Product Information:
Product Information
- Volume : 1 mL (100 rxn)
Description
- This product is supplied as a ready-to-use reagent for the measurement of relative cell number. Reduction of the water-soluble tetrazolium salt WST-8 by the cell membrane-permeable electron carrier 1-MePMS converts it to a highly colored form which can be detected using a plate reader fitted with a 460 nm absorbance filter. As 1-MePMS is itself reduced by intracellular NADH/NADPH, and NADH/NADPH concentration is relatively constant over the course of the cell cycle, the amount of reduced WST-8 and therefore the degree of color change can be used to determine relative cell number,
Direction for Use
-
- Prepare cell samples in a 96 well plate (do not remove medium).
- Apply 10 µL of W0023 to each well and incubate for up to 4 hours* at 37°C / 5% CO2.
- Measure the absorbance at 460 nm.
- *Optimal incubation times should be determined for each individual cell line
Storage
- Store at 4°C. Use within 6 months of receipt.
| Product Number | W0023 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Light red to Red clear liquid |
| Cell Number-to-Signal Linearity Test | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Product Documents (Note: Some products will not have analytical charts available.)
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Specifications
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