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Product Information

Volume/Quantity : 2 mg
Product Form : Suspension in water
Functional Group : Thiol group (-SH)
Excitation / Emission : 556 nm / 579 nm

Application

For use as a fluorophore in the fluorescent labeling of proteins and other molecules. Incorporation of Rhodamine B fluorescent dye into conjugatable silica nanoparticles allows for superior fluorescence intensity and light resistance when compared to unmodified Rhodamine B. Thiol groups (-SH) at the surface of the nanoparticles provide a convenient mechanism for conjugation. This product is made without toxic metals.

Storage

Store at 4 °C. Do not freeze.

Directions for Us

Usage Example:
Conjugation with Mouse IgG antibody (M.W. 150,000)

1. Prepare 10 mM linker solution (For examples of linkers, see Related Products). e.g. Dissolve 2.8 mg of GMBS (Product No. S0399) in 1 mL of DMSO (Product No. D0798).
2. Mix 10 mM linker solution with appropriate volume of antibody. (Suggested linker:antibody molar ratio = 100:1) e.g. Mix 100 μL of 1.5 mg/mL (= 10 μM) antibody solution with 10 μL of 10 mM linker solution.
3. Incubate linker-antibody solution for 1 hour at room temperature.
4. Remove unreacted and/or hydrolyzed reagent using a desalting column or dialysis method (not included).
5. Mix 1 part by weight purified antibody-linker solution with 1.5 parts by weight Organosilica Rhodamine B (Product No. O0573). e.g. For the antibody-linker solution described in step 2 (antibody weight approx. 0.15 mg), use 45 μL of 5.0 mg/mL Organosilica Rhodamine B.
6. Incubate the antibody-nanoparticle solution for 1 hour at room temperature.
7. Collect conjugated nanoparticles via centrifugation (20,000 x g, 4 °C, 10 min) and wash with 500 μL deionized water. Repeat wash step two additional times. Resuspend final washed pellet in deionized water.

Related Products

Linkers for Thiol-Amine Conjugation

S0399 N-Succinimidyl 4-Maleimidobutyrate
S0427 N-Succinimidyl 3-Maleimidopropionate
S0861 N-Succinimidyl 4-(4-Maleimidophenyl)butyrate
S0882 N-Succinimidyl 11-Maleimidoundecanoate

Related special Fluorescent Products

O0561 Organosilica FITC 100nm
B5419 BMeS-p-A : Extremely small fluorescent dye