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CAS RN: 601494-52-4 | 제품번호: A2083
ATBTA-Eu3+
![ATBTA-Eu3+ No-Image](/medias/A2083.jpg?context=bWFzdGVyfHJvb3R8NjM0Nzl8aW1hZ2UvanBlZ3xhREkxTDJoaE5TODRPVEU1TmpRMk5UTTFOekV3TDBFeU1EZ3pMbXB3Wnd8ZDVlMjAxOWY2NzhiOTYwMzhhYTRjNGY5ZDdlOTU1ZTk1NzE1MDk0NDgyOWI2Y2RiOWNlOTRhNDIxMTQ5NThiYw)
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•보관 조건은 예고없이 변경 될 수 있습니다. 제품 보관 조건의 최신 자료는 홈페이지에 기재되어 있으니 양해 부탁드립니다.
•본건의 원가격은 한국 대리점의 예상 판매가격입니다.자세한 정보가 필요하시면 연락해 주십시오.( SEJIN CI Co., Ltd. (한국총대리점) 전화 : 02-2655-2480 이메일 : sales@sejinci.co.kr)
•보관 조건은 예고없이 변경 될 수 있습니다. 제품 보관 조건의 최신 자료는 홈페이지에 기재되어 있으니 양해 부탁드립니다.
제품번호 | A2083 |
Purity/Analysis Method | >90.0%(T)(HPLC) |
M.F. / M.W. | C__3__7H__3__0EuN__6NaO__8 = 861.63 |
물리적 상태 (20 ℃) | Solid |
보관 조건 | Room Temperature (Recommended in a cool and dark place, <15°C) |
불활성 가스 하에서 보관 | Store under inert gas |
피해야 할 조건 | Light Sensitive,Air Sensitive |
용기 | 10MG-Glass Bottle with Plastic Insert (이미지 보기) |
CAS RN | 601494-52-4 |
PubChem Substance ID | 87560385 |
MDL 번호 | MFCD10566875 |
규격표
Appearance | Light yellow to Brown to Dark green powder to crystaline |
Purity(HPLC) | min. 90.0 area% |
Purity(Chelometric Titration) | min. 90.0 % |
Water | max. 10.0 % |
물성치(참고치)
Solubility in water | Soluble |
GHS
법규 정보
운송 정보
HS 번호* | 2846.90-000 |
Application
DTBTA-Eu3+ Labeling of proteins
References
- Sodium Dodecyl Sulfate Polyacrylamide Slab Gel Electrophoresis and Hydroxyethyl Cellurose Gel Capillary Electrophoresis of Luminescent Lanthanide Chelate-labeled Proteins with Time-Resolved Detection
Application
Europium Fluorescent Labeling Reagent suitable for Time-resolved Fluorometry
ATBTA-Eu3+ is a europium chelate complex and can be used as a fluorescent labeling reagent. ATBTA-Eu3+ is easily labeled to protein etc. after conversion to DTBTA-Eu3+.
The new lanthanide chelate reagent, DTBTA-Eu3+, has a high stability constant, and therefore the problem of fluorescence intensity change in different buffers has been greatly reduced. DTBTA-Eu3+ has also several advantages such as the intensity stability in water for a long period, and the stability against photo-bleaching.
■Typical Procedure: Preparation of DTBTA-Eu3+
Dissolve 2mg of ATBTA-Eu3+ [A2083] in 60µL of 0.1M acetate buffer (pH4.9). This solution is added 0.43mg of Cyanuric Chloride [C0460] in 25µL of acetone, and stirred for 30 min. The reaction mixture is added dropwise to 1mL of acetone, and formed precipitate is centrifuged. After washing with 0.5mL of acetone twice, the yellow powder is dried in vacuum for 1 h. Dissolve the powder in 1mL of carbonate buffer gives (pH9) for labeling. This solution contains ca. 2mM of labeling reagent.
■Warning
This labeling reagent is deactivated by hydrolysis, especially in alkali solution. The reagent dissolved in water should be used immediately. For temporary storage, the reagent should be dissolved in buffer solution at acidic pH (pH~5) and kept at 0°C.
Please refer to “Anti-DTBTA-Eu3+ Antibody” for the application of the DTBTA-Eu3+ label.
■Typical Procedure: Preparation of DTBTA-Eu3+
Dissolve 2mg of ATBTA-Eu3+ [A2083] in 60µL of 0.1M acetate buffer (pH4.9). This solution is added 0.43mg of Cyanuric Chloride [C0460] in 25µL of acetone, and stirred for 30 min. The reaction mixture is added dropwise to 1mL of acetone, and formed precipitate is centrifuged. After washing with 0.5mL of acetone twice, the yellow powder is dried in vacuum for 1 h. Dissolve the powder in 1mL of carbonate buffer gives (pH9) for labeling. This solution contains ca. 2mM of labeling reagent.
■Warning
This labeling reagent is deactivated by hydrolysis, especially in alkali solution. The reagent dissolved in water should be used immediately. For temporary storage, the reagent should be dissolved in buffer solution at acidic pH (pH~5) and kept at 0°C.
Please refer to “Anti-DTBTA-Eu3+ Antibody” for the application of the DTBTA-Eu3+ label.
References
- New Luminescent Europium(III) Chelates for DNA Labeling
- 4-Hydroxynonenal modifies IgA in rat intestine after lipopolysaccharide injection
- Application of a fluorescent lanthanide chelate label on a solid support device for detecting DNA variation with ligation-based assay
- A novel sensitive immunoassay method based on the Invader technique
PubMed Literature
기사 / 브로셔
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